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Gene expression pattern in the adult brain of the experimental rat after prenatal exposure to methamphetamine
Tomášková, Anežka ; Černá, Marie (advisor) ; Rudá, Jana (referee)
Introduction: Methamphetamine is a drug frequently taken by drug-addicted pregnant women and happens to be one of the most commonly used drugs in the Czech Republic. This drug passes easily through a placental barrier into the fetus. Thus it can negatively affect not only the mother but also the prenatal development of her offspring. Objectives: This research aims to provide a general screening of gene expression in selected regions of the F1 generation of the brain prenatally affected by methamphetamine, to verify whether exposure to methamphetamine affects the generation of offspring of exposed females at the level of gene expression in selected regions of the brain, and to valuate possible changes in gene expression. Methods: In selected parts of the brain, collected from a rat, the microarray hybridization and the real-time PCR were set to evaluate express changes in the expression of selected genes. Results: Statistical analysis of the microarray hybridization did not show a significantly altered gene expression in the tested genes. Only boundary values for 13 genes were measured, which were further tested by the real-time PCR. After a statistic evaluation of the real-time PCR, the significantly altered expression was found in 2 genes. The notably changed expression of DRD3 and TACR3 genes...
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Gene expression of specific parts of CNS in a rat model for transgenerational transmission of drug addiction.
Radilová, Kateřina ; Černá, Marie (advisor) ; Rothová, Olga (referee)
Methamphetamine (MA) is a drug often abused by drug-addicted women and also is the mostly used drug in the Czech Republic. This drug crosses the placental and hematoencephalic barriers easily and therefore it can negatively affect not just mother herself, but also the development of the fetus. Even though there have been several published studies evincive negative effects of MA to posterity of drug-addicted mothers, the most critical term during pregnancy for the fetus remains unknown. In this study are examined CNS structures, which are directly influenced by methamphetamine action. Their function may lead to drug addiction through pleasurable feelings, such as reward feeling evoking. In the next chapter are analysed neurotransmitters and their transporters, which are related to methamphetamine effects. Studying their structure, localization or development during life may help to bring near the drug functioning in different life periods. Methamphetamine itself and its effects are closer described in the last part of this study. The problem of methamphetamine exposed fetus is outlined there - prenatal and postnatal so far known differences, but also sex differences in methamphetamine pharma- cokinetics.
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Gene expression pattern in the adult brain of the experimental rat after prenatal exposure to methamphetamine
Tomášková, Anežka ; Černá, Marie (advisor) ; Rudá, Jana (referee)
Introduction: Methamphetamine is a drug frequently taken by drug-addicted pregnant women and happens to be one of the most commonly used drugs in the Czech Republic. This drug passes easily through a placental barrier into the fetus. Thus it can negatively affect not only the mother but also the prenatal development of her offspring. Objectives: This research aims to provide a general screening of gene expression in selected regions of the F1 generation of the brain prenatally affected by methamphetamine, to verify whether exposure to methamphetamine affects the generation of offspring of exposed females at the level of gene expression in selected regions of the brain, and to valuate possible changes in gene expression. Methods: In selected parts of the brain, collected from a rat, the microarray hybridization and the real-time PCR were set to evaluate express changes in the expression of selected genes. Results: Statistical analysis of the microarray hybridization did not show a significantly altered gene expression in the tested genes. Only boundary values for 13 genes were measured, which were further tested by the real-time PCR. After a statistic evaluation of the real-time PCR, the significantly altered expression was found in 2 genes. The notably changed expression of DRD3 and TACR3 genes...
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Prenatal exposure to methamphetamine and its effect on the gene expression in the selected parts of the brains of experimental rats
Tomášková, Anežka ; Zoubková, Hana (advisor) ; Rudá, Jana (referee)
Introduction: Methamphetamine is a drug frequently abused by drug-addicted pregnant women and also one of the mostcommonly used drugs in the CzechRepublic. This drug passes easily through a placental barrier into the fetus. Thus it can negatively affect not only the mother but also the prenatal development of her offspring. Objectives: In the framework of the grant project GA CR: 14-03708S, the long-term effects of prenatal exposure to methamphetamine were detected. It was determined whether the prenatal methamphetamine exposure affects the generation of offspring of exposed females at the level of gene expression of genes in specific regions of the brain, striatum, hippocampus and prefrontal cortex. Methods: In the selected parts of the brain, which were removed from the rat, the microarray hybridization and the real-time PCR to express changes in expression of selected genes were performed. Results: Statistical analysis of microarray hybridization did not show the significantly altered gene expression in tested genes significantly. Only boundary values for 13 genes were measured, which were further tested by real-time PCR.After a statistic evaluation of real-time PCR, the significantly altered expression was found in 2 genes. The significantly changed expression of DRD3 and TACR3 genes was found...
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Gene expression of specific parts of CNS in a rat model for transgenerational transmission of drug addiction.
Radilová, Kateřina ; Černá, Marie (advisor) ; Rothová, Olga (referee)
Methamphetamine (MA) is a drug often abused by drug-addicted women and also is the mostly used drug in the Czech Republic. This drug crosses the placental and hematoencephalic barriers easily and therefore it can negatively affect not just mother herself, but also the development of the fetus. Even though there have been several published studies evincive negative effects of MA to posterity of drug-addicted mothers, the most critical term during pregnancy for the fetus remains unknown. In this study are examined CNS structures, which are directly influenced by methamphetamine action. Their function may lead to drug addiction through pleasurable feelings, such as reward feeling evoking. In the next chapter are analysed neurotransmitters and their transporters, which are related to methamphetamine effects. Studying their structure, localization or development during life may help to bring near the drug functioning in different life periods. Methamphetamine itself and its effects are closer described in the last part of this study. The problem of methamphetamine exposed fetus is outlined there - prenatal and postnatal so far known differences, but also sex differences in methamphetamine pharma- cokinetics.
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Effect of addictive drugs on prenatal development
Hofmannová, Oldřiška ; Peterka, Miroslav (advisor) ; Peterková, Renata (referee)
Substance abuse is long-term worldwide problem. This thesis summarizes findings about the effects of stimulants, opiates and hallucinogens and focuses on the most commonly used illegal representatives of individual groups: cocaine, amphetamines, heroin and LSD. All these substances have negative effect on both mother and her fetus. Some of addictive substances can influence pregnancy and childbirth. There is also higher risk of fetus mortality and morbidity of the new born baby. Prenatal exposure to addictive substances could have influence on the birth weight, length, head circumference, its further growth and behavior. Some drugs can cause neonatal abstinent syndrome which can lead without prenatal care to death of newborn.
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